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Ribosomal incorporation of cyclic β-amino acids into peptides using in vitro translation

https://doi.org/10.1039/D0CC02121K

Lee, Joongoo ; Torres, Rafael ; Kim, Do Soon ; Byrom, Michelle ; Ellington, Andrew D. ; Jewett, Michael C. ( May 2020 , Chemical Communications)

We demonstrate in vitro incorporation of cyclic β-amino acids into peptides by the ribosome through genetic code reprogramming. Further, we show that incorporation efficiency can be increased through the addition of elongation factor P.
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Expanding the limits of the second genetic code with ribozymes

https://doi.org/10.1038/s41467-019-12916-w

Lee, Joongoo ; Schwieter, Kenneth E. ; Watkins, Andrew M. ; Kim, Do Soon ; Yu, Hao ; Schwarz, Kevin J. ; Lim, Jongdoo ; Coronado, Jaime ; Byrom, Michelle ; Anslyn, Eric V. ; et al ( December 2019 , Nature Communications)

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